Journal: Food Science & Nutrition

Article Title: Hydroxysafflor yellow A, a natural food pigment, ameliorates atherosclerosis in ApoE −/− mice by inhibiting the SphK1 / S1P / S1PR3 pathway

doi: 10.1002/fsn3.4466

Figure Lengend Snippet: Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL staining and quantitative analysis. Scale bar: 200 μm. (e, f) Evans blue staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.

Article Snippet: One hour before execution, mice were injected with 0.5% 2 mL/kg Evans blue staining solution (Macklin, Shanghai, China) in the tail vein.

Techniques: Permeability, Immunohistochemical staining, Expressing, TUNEL Assay, Staining

Journal: Scientific Reports

Article Title: Ameliorative effects of melatonin on dark-induced leaf senescence in gardenia ( Gardenia jasminoides Ellis): leaf morphology, anatomy, physiology and transcriptome

doi: 10.1038/s41598-017-10799-9

Figure Lengend Snippet: Effect of 1.0 mM melatonin on programmed cell death during dark-induced gardenia leaf senescence. (A) PCD detection by staining with Evans blue solution. Cell death is indicated by cells stained blue and marked by a red arrow. (B) PCD detection by the isolated mesophyll cell observation.

Article Snippet: The samples were stained with 0.1% Evans blue staining solution (Shanghai Yuanye biological technology Co., Ltd., China) for 2 min and then washed with double-distilled water.

Techniques: Staining, Isolation